Figure 3: Intrinsic excitability of neurons located in the contralateral M1 in Layer 2/3 and Layer 5/6 was detected by slice electrophysiological recording within 24 hours of unilateral focal photothrombotic infarct in M1. In Layer2/3 neurons, the resting membrane potential (Resting MP) (A1), input resistance (Rin) (A2), threshold to spike (A3) and spike number induced at 300 pA current injection (Spike #) (A4) were not significantly different between the sham, stroke, and stroke + EA groups. Number of neurons = 9, 12, and 8 in the sham, stroke and stroke + EA groups, respectively. (B1–B4) Similar to the results in Layer 2/3, the resting membrane potential (B1), input resistance (B2), threshold to spike (B3), and spike number induced at 300pA current injection (B4) in Layer 5/6 neurons were not affected by stroke or EA. All experiments were repeated five times. Data are presented as means ± standard error of the mean (see [Table 1] for the number of mice used in [Figure 3]). Number of neurons = 14, 15, and 10 in the sham, stroke, and stroke + EA groups, respectively. EA: Electroacupuncture; L2/3: Layer 2/3; L5/6: Layer 5/6.