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  Indian J Med Microbiol
 

Figure 6: Lithium attenuates inflammatory, oxidative, and pyroptotic injury in PC12 cells subjected to OGD. Con group: untreated PC12 cells; OGD group: PC12 cells subjected to OGD; OGD + LiCl group: PC12 cells subjected to OGD and treated with LiCl; OGD + LiCl + ATRA group: PC12 cells subjected to OGD and treated with LiCl and the Nrf2 inhibitor ATRA. (A–I) TNF-α (A), IL-1β (B), IL-6 (C), CAT (D), GSH-Px (E), LDH (F), SOD (G), MDA (H), and T-AOC (I) expression as detected by ELISA. (J–N) Flow cytometry analysis of the percentage of pyroptotic PC12 cells. Data are shown as mean ± SD (n = 6). *P < 0.05, vs. control group; #P < 0.05, vs. OGD group; &P < 0.05, vs. OGD + LiCl group (one-way analysis of variance followed by least significant difference test). ATRA: All-trans retinoic acid; CAT: catalase; Con: control; GSH-Px: glutathione peroxidase; IL-1β: interleukin-1β; IL-6: interleukin-6; LiCl: lithium chloride; LPO: lipid peroxide; MDA: malondialdehyde; OGD: oxygen glucose deprivation; SOD: superoxide dismutase; T-AOC: total antioxidant capacity; TNF-α: tumor necrosis factor-alpha.

<b>Figure 6: Lithium attenuates inflammatory, oxidative, and pyroptotic injury in PC12 cells subjected to OGD.</b>
Con group: untreated PC12 cells; OGD group: PC12 cells subjected to OGD; OGD + LiCl group: PC12 cells subjected to OGD and treated with LiCl; OGD + LiCl + ATRA group: PC12 cells subjected to OGD and treated with LiCl and the Nrf2 inhibitor ATRA. (A–I) TNF-α (A), IL-1β (B), IL-6 (C), CAT (D), GSH-Px (E), LDH (F), SOD (G), MDA (H), and T-AOC (I) expression as detected by ELISA. (J–N) Flow cytometry analysis of the percentage of pyroptotic PC12 cells. Data are shown as mean ± SD (<i>n</i> = 6). *<i>P</i> < 0.05, <i>vs</i>. control group; #<i>P</i> < 0.05, <i>vs</i>. OGD group; &<i>P</i> < 0.05, <i>vs</i>. OGD + LiCl group (one-way analysis of variance followed by least significant difference test). ATRA: All-trans retinoic acid; CAT: catalase; Con: control; GSH-Px: glutathione peroxidase; IL-1β: interleukin-1β; IL-6: interleukin-6; LiCl: lithium chloride; LPO: lipid peroxide; MDA: malondialdehyde; OGD: oxygen glucose deprivation; SOD: superoxide dismutase; T-AOC: total antioxidant capacity; TNF-α: tumor necrosis factor-alpha.