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  Indian J Med Microbiol
 

Figure 2: Effect of combination of myelin-associated glycoprotein and chitin conduit on the pathology of regenerated nerve fibers in proximal nerve stumps 8 weeks postoperatively. (A) Masson’s trichrome staining of cross sections of the proximal nerve stump. Collagen fibers are shown in blue and nerve fibers in pink. More collagen fibers (black arrow) were observed in the model group, and regular distributed nerve fibers (red arrow) were observed in the combination group. (B) Quantitative results of the percentage of collagen fiber area. (C) α-SMA immunohistochemical staining (brown) of cross sections of the proximal nerve stump. In the conduit and combination groups, α-SMA was mainly expressed in the walls of blood vessels (red arrow). (D) Quantitative results of the percentage of α-SMA positive staining area. (E) NF200 immunofluorescence staining of cross sections of the proximal nerve stump. The fluorescent indicator used was Alexa Fluor 594 for NF200 (red). Nuclei are shown in blue. Little axons (red arrow) were observed in the combination group. Scale bars: 50 μm in A, 100 μm in C and E. (F) Quantitative results of the density of regenerated axons. The axon density was defined as the number of axons within an area of 5000 μm2. Data are expressed as the mean ± SD (n = 5 animals per group). ##P < 0.01, vs. model group; **P < 0.01, vs. conduit group (one-way analysis of variance followed by Tukey’s post hoc test). NF200: Neurofilament-200; α-SMA: alpha-smooth muscle actin.

<b>Figure 2: Effect of combination of myelin-associated glycoprotein and chitin conduit on the pathology of regenerated nerve fibers in proximal nerve stumps 8 weeks postoperatively.</b>
(A) Masson’s trichrome staining of cross sections of the proximal nerve stump. Collagen fibers are shown in blue and nerve fibers in pink. More collagen fibers (black arrow) were observed in the model group, and regular distributed nerve fibers (red arrow) were observed in the combination group. (B) Quantitative results of the percentage of collagen fiber area. (C) α-SMA immunohistochemical staining (brown) of cross sections of the proximal nerve stump. In the conduit and combination groups, α-SMA was mainly expressed in the walls of blood vessels (red arrow). (D) Quantitative results of the percentage of α-SMA positive staining area. (E) NF200 immunofluorescence staining of cross sections of the proximal nerve stump. The fluorescent indicator used was Alexa Fluor 594 for NF200 (red). Nuclei are shown in blue. Little axons (red arrow) were observed in the combination group. Scale bars: 50 μm in A, 100 μm in C and E. (F) Quantitative results of the density of regenerated axons. The axon density was defined as the number of axons within an area of 5000 μm<sup>2</sup>. Data are expressed as the mean ± SD (<i>n</i> = 5 animals per group). ##<i>P</i> < 0.01, <i>vs</i>. model group; **<i>P</i> < 0.01, <i>vs</i>. conduit group (one-way analysis of variance followed by Tukey’s <i>post hoc</i> test). NF200: Neurofilament-200; α-SMA: alpha-smooth muscle actin.