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  Indian J Med Microbiol
 

Figure 3: Comparing optical tissue clearing and the conventional cryosection technique for the quantitative assessment of retrogradely labeled common peroneal nerve neurons. (A) Processing steps for fixed tissue in both techniques, indicating substantial time savings when tissue clearing is used compared to cryosections. (B) Comparing the number of motor and sensory neurons derived from optically cleared (orange) and cryosectioned tissue (grey), showing that significantly more neurons were identified in intact, cleared spinal cord and DRGs. (C) The number of labeled common peroneal nerve sensory neurons that have been identified by both techniques in the dorsal root ganglia from L1 to L6. The results indicate a tendency of the conventional technique to overestimate cell counts in ganglia with few labeled neurons (L2 and L3) and to underestimate neuron counts in ganglia with a high density of labeled cells (L4 and L5), although the differences did not reach statistical significance in this experiment (P > 0.05). DBE: Dibenzyl ether; DCM: dichloromethane; DRG: dorsal root ganglia; ETOH: ethanol; THF: tetrahydrofuran.

<b>Figure 3: Comparing optical tissue clearing and the conventional cryosection technique for the quantitative assessment of retrogradely labeled common peroneal nerve neurons.</b>
(A) Processing steps for fixed tissue in both techniques, indicating substantial time savings when tissue clearing is used compared to cryosections. (B) Comparing the number of motor and sensory neurons derived from optically cleared (orange) and cryosectioned tissue (grey), showing that significantly more neurons were identified in intact, cleared spinal cord and DRGs. (C) The number of labeled common peroneal nerve sensory neurons that have been identified by both techniques in the dorsal root ganglia from L1 to L6. The results indicate a tendency of the conventional technique to overestimate cell counts in ganglia with few labeled neurons (L2 and L3) and to underestimate neuron counts in ganglia with a high density of labeled cells (L4 and L5), although the differences did not reach statistical significance in this experiment (<i>P</i> > 0.05). DBE: Dibenzyl ether; DCM: dichloromethane; DRG: dorsal root ganglia; ETOH: ethanol; THF: tetrahydrofuran.