Figure 5: Cutaneous innervation in the rat sural nerve dermatome. (A) A full-thickness skin graft from the dorsum of the fifth toe was harvested, immunostained against the neuronal marker beta-3-tubulin (red) and optically cleared for fluorescence imaging. (B) Cutaneous nerve branches in higher magnification. (C) Segmentation of the cutaneous innervation as an overlay/merge with the raw image shown in B. (D) Nerve fiber segmentation only. (E) Cutaneous nerve branches with free nerve endings in high magnification. The histogram in (F) displays the percentage frequency of the branching levels of the cutaneous nerve plexus, after compartmentalization into inter-branch segments. The branching level describes the number of branch points proximal to a segment. (G) The percentage frequency of the average diameter of a nerve branch. Scale bars: 1 mm in A, 200 μm in B, 200 μm in C, D, 50 μm in E. Q: Quartile.