Figure 3: Immunostaining of adjacent longitudinal sections of the same injured spinal cord grafted with donor hMSCs, examined at D56 (49 days post-transplantation) revealing neuronal marker (βIII tubulin+, GAP43+ and SMI32+ fiber) profiles in injury sites. Neuronal marker (βIII tubulin+, GAP43+ and SMI32+ fiber) profiles in injury sites appeared to be denser and more numerous in hMSC treated animals, but lacked specific orientation and organization. STEM101 marker (HNA, red) co-stained with βIII tubulin+ (green) axons that fill the lesion site (A1, B1); numerous GAP43+ (red; A2, B2) and SMI32 fibers (red; A3, B3) surrounded and extended through hMSC graft areas. Scale bars: 200 μm. GAP43: growth associated protein-43; hMSCs: human mesenchymal stromal cells; HNA: human nuclear antigen.