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  Indian J Med Microbiol
 

Figure 4: Immunostaining of adjacent longitudinal sections of the same injured spinal cord grafted with donor hMSCs examined at D56 (49 days post-transplantation) revealing astrocytic (GFAP+), extracellular matrix (laminin+) and microglial/macrophage (ED1+) marker profiles in injury sites. Intense GFAP immunoreactivity restricted at the hMSC graft border had very few GFAP+ processes extending into the graft, whilst laminin deposits and ED1+ cells often filled and bridged the lesion site, extending between uninjured rostral and caudal tissue. STEM101 marker (HNA; red) co-stained with GFAP (green; A1, B1), laminin (green; A2, B2) and ED1 (red; A3, B3) throughout hMSC graft areas. Scale bars: 200 μm. GFAP: Glial fibrillary acidic protein; hMSCs: human mesenchymal stromal cells; HNA: human nuclear antigen.

<b>Figure 4: Immunostaining of adjacent longitudinal sections of the same injured spinal cord grafted with donor hMSCs examined at D56 (49 days post-transplantation) revealing astrocytic (GFAP<sup>+</sup>), extracellular matrix (laminin<sup>+</sup>) and microglial/macrophage (ED1<sup>+</sup>) marker profiles in injury sites.</b>
Intense GFAP immunoreactivity restricted at the hMSC graft border had very few GFAP<sup>+</sup> processes extending into the graft, whilst laminin deposits and ED1<sup>+</sup> cells often filled and bridged the lesion site, extending between uninjured rostral and caudal tissue. STEM101 marker (HNA; red) co-stained with GFAP (green; A1, B1), laminin (green; A2, B2) and ED1 (red; A3, B3) throughout hMSC graft areas. Scale bars: 200 μm. GFAP: Glial fibrillary acidic protein; hMSCs: human mesenchymal stromal cells; HNA: human nuclear antigen.