Figure 4: Immunostaining of adjacent longitudinal sections of the same injured spinal cord grafted with donor hMSCs examined at D56 (49 days post-transplantation) revealing astrocytic (GFAP+), extracellular matrix (laminin+) and microglial/macrophage (ED1+) marker profiles in injury sites. Intense GFAP immunoreactivity restricted at the hMSC graft border had very few GFAP+ processes extending into the graft, whilst laminin deposits and ED1+ cells often filled and bridged the lesion site, extending between uninjured rostral and caudal tissue. STEM101 marker (HNA; red) co-stained with GFAP (green; A1, B1), laminin (green; A2, B2) and ED1 (red; A3, B3) throughout hMSC graft areas. Scale bars: 200 μm. GFAP: Glial fibrillary acidic protein; hMSCs: human mesenchymal stromal cells; HNA: human nuclear antigen.